#bead beater homogenizer

[mpbiomedicals]

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[interninggenetics]

Week 1

My first week as an intern at Genetic ID was a multifaceted introduction, as I was greeted by the seven person crew, a list of new terms to study, and an array of equipment to understand.  I learned protocols of good “bench chemistry”, which is the active form of conducting research experiments, compared to manufacturing or sales.  My main job was to assist in sample preparation, which included homogenizing food products by mixing them up via VitaMix blenders, hand and spoon, or with a knife and cutting board. The method of homogenization is dependent on the sample. For example, if a company sent in loaves of bread to be tested, we would dice them. For a corn kernel sample we would use a blender, but for something with fine particles like flour, we would mix it by hand with a spoon. The food products would then be transported into three test tubes; one for the laboratory’s archive and two to be tested for DNA. The most common products we received were corn kernels, soy flakes, and animal feed, but we also received protein powder, canola oil and honey.  Companies send in food products to Genetic ID mainly for GMO testing, and for certain animal particles in livestock feed to prevent diseases caused by animals eating their own species, like bovine encephalitis and scrapies. The presence of encephalitis in food can cause a disease in humans called creutzfeld jacobs, which slowly degenerates the brain, killing most patients within a year. 

The most exciting thing I learned this week was the process of DNA extraction. I was lucky enough to shadow a scientist who was extracting DNA from pollen in honey. The company sent in the sample in order to see whether their bees were pollenating with GMO plants. DNA extraction for a substance like honey begins with diluting it with deionized water in order to make the honey less dense, and it is also spun on a wheel for about half an hour to ensure that the water is thoroughly incorporated. Next the substance is put through a process called centrifugation, which separates substances based on density.  In this case, the aim was to separate the pollen from the honey. The centrifugation machine spun the test tubes in a horizontal position at a speed of 3500 RPMs for 30 minutes.  The scientist was surprised at the large amount of pollen we received from this process, stating the it looked to be five times more than usual. We then transported the pollen to smaller vials that had a ceramic bead and grains of sand, which was put into a “bead beater” machine that was meant to finely break up the pollen. Then we added three enzymes to the vials that were meant to purify our sample. Ribonuclease (RNAse) was the enzyme to rid our sample of any RNA. The Proteinase K was used to eat away at the proteins. Lysis was added to disrupt cell tissue, and open the cells’ nucleus. The sample was then transported to an incubator with a temperature of 65 degrees Fahrenheit, in order to encourage the enzymes to activate.  After this the scientist had an urgent duty to attend to and had to end the tutorial, but I will hopefully be shadowing other extractions very soon.

[mpbiomedicals]

Enhancing Sample Preparation Efficiency with Bead Beater Machines

Discover how bead beater machines revolutionize sample preparation, ensuring efficient cell lysis and homogenization. Explore the benefits of bead beater machines and their role in streamlining various life science research processes. Learn more at MP Bio's website.